abstract：BACKGROUND AIMS:Enumeration of CD34(+) cells in leukocyte-rich cell suspensions is important for clinical decision-making in stem cell transplantation. Single-platform flow cytometry assays offer the significant advantages of speed and reproducibility, and have therefore become the gold standard in stem cell enumeratio...

journal_title：Cytotherapy

authors： Dauber K,Becker D,Odendahl M,Seifried E,Bonig H,Tonn T

更新日期：2011-04-01 00:00:00

abstract：BACKGROUND AIMS:Shipment of therapeutic somatic cells between a current good manufacturing practice (cGMP) facility and a clinic or between different cGMP facilities requires validated standard operating procedures (SOP). Under National Heart Lung & Blood Institute (NHLBI) sponsorship, the Production Assistance for Cel...

journal_title：Cytotherapy

authors： Whiteside TL,Griffin DL,Stanson J,Gooding W,McKenna D,Sumstad D,Kadidlo D,Gee A,Durett A,Lindblad R,Wood D,Styers D

更新日期：2011-02-01 00:00:00

abstract：BACKGROUND AIMS:Recent evidence has suggested that transplanted bone marrow (BM)-derived mesenchymal stromal cells (MSC) are able to engraft and repair non-hematopoietic tissues successfully, including central nervous system, renal, pulmonary and skin tissue, and may possibly contribute to tissue regeneration. We exami...

journal_title：Cytotherapy

authors： Jung EJ,Kim SC,Wee YM,Kim YH,Choi MY,Jeong SH,Lee J,Lim DG,Han DJ

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND AIMS:Chimerism is an important outcome measure in hematopoietic cell transplantation as well as somatic cell therapy. Commonly used methods to estimate chimerism are restricted by either gender or inefficient sensitivity. In principle, real-time polymerase chain reaction (PCR)-based assays can be used to ass...

journal_title：Cytotherapy

authors： Chen X,Hofmann TJ,Otsuru S,Jethva R,Lind C,Monos D,Horwitz EM

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) are gaining in popularity as an experimental therapy for a number of conditions that often require expansion ex vivo prior to use. Data comparing clinical-grade MSC from various ages of donors are scant. We hypothesized that MSC from older donors may display differences i...

journal_title：Cytotherapy

authors： Lund TC,Kobs A,Blazar BR,Tolar J

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND AIMS:Circulating monocytes have been exploited as an important progenitor cell resource for hepatocytes in vitro and are instrumental in the removal of fibrosis. We investigated the significance of monocytes in peripheral blood stem cells (PBSC) for the treatment of liver cirrhosis. METHODS:Rat CD14+ monocy...

journal_title：Cytotherapy

authors： Wang J,Zhou X,Cui L,Yan L,Liang J,Cheng X,Qiao L,Shi Y,Han Z,Cao Y,Han Y,Fan D

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND AIMS:Tumor antigen-specific cytotoxic T lymphocytes (CTL) have been used in the treatment of human cancer, including leukemia. Several studies have established PR1 peptide, an HLA-A2.1-restricted peptide derived from proteinase 3 (P3), as a human leukemia-associated antigen. PR1-specific CTL elicited in vitr...

journal_title：Cytotherapy

authors： Ma Q,Wang C,Jones D,Quintanilla KE,Li D,Wang Y,Wieder ED,Clise-Dwyer K,Alatrash G,Mj Y,Munsell MF,Lu S,Qazilbash MH,Molldrem JJ

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND AIMS:The beneficial effect of human (h) mesenchymal stromal cell (MSC) transplantation in a variety of cell-based intervention strategies is widely believed to be because of paracrine mechanisms. The modification of hMSC cytokine and growth-factor expression patterns were studied following exposure to lipopo...

journal_title：Cytotherapy

authors： Montzka K,Führmann T,Müller-Ehmsen J,Wöltje M,Brook GA

更新日期：2010-11-01 00:00:00

abstract：BACKGROUND AIMS:The aim was to investigate the therapeutic effect of granulocyte-colony-stimulating factor (G-CSF) administration following implantation of autologous bone marrow mononuclear cells (BM MNC) for patients with lower limb ischemia. METHODS:The design was a randomized controlled trial. Fifteen patients wit...

journal_title：Cytotherapy

authors： Zafarghandi MR,Ravari H,Aghdami N,Namiri M,Moazzami K,Taghiabadi E,Fazel A,Pournasr B,Farrokhi A,Sharifian RA,Salimi J,Moini M,Baharvand H

更新日期：2010-10-01 00:00:00

abstract：BACKGROUND AIMS:Cytotherapy is a promising option for neurodegenerative disease treatment. Because of the fatal prognosis and imperative need for effective treatment, amyotrophic lateral sclerosis (ALS) patients request this therapy before its effectiveness has been verified. The increase in clinics offering cytotherap...

journal_title：Cytotherapy

authors： Gamez J,Carmona F,Raguer N,Ferrer-Sancho J,Martín-Henao GA,Martí-Beltrán S,Badia M,Gratacós M,Rodriguez-Gónzalez E,Seoane JL,Pallero-Castillo M,Burgos R,Puiggros C,Pasarin A,Bori-Fortuny I

更新日期：2010-09-01 00:00:00

abstract：BACKGROUND AIMS:Stem cells derived from human adipose tissue (ASC) have the capacity for renewal, are easily obtained and have plasticity properties that allow them to differentiate into several cell types, including osteoblast cells. With the aim of understanding the issue of the osteogenic process and finding reliabl...

journal_title：Cytotherapy

authors： Giusta MS,Andrade H,Santos AV,Castanheira P,Lamana L,Pimenta AM,Goes AM

更新日期：2010-07-01 00:00:00

abstract：:A 3-year-old girl with severe aplastic anemia (SAA) that was unresponsive to steroid, cyclosporine and filgrastim treatments received bone marrow (BM) mesenchymal stromal cells (MSC; 1.25 x 10(6)/kg), granulocyte colony-stimulating factor (G-CSF)-mobilized BM and peripheral blood stem cell grafts from her father. Prio...

journal_title：Cytotherapy

authors： Wang H,Wang Z,Xue M,Liu J,Yan H,Guo Z

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND AIMS:In recent years, bone marrow (BM)-derived mesenchymal stromal cells (MSC) have become a promising source for neuroregenerative therapies. We evaluated the trophic effects of neuroectodermally converted MSC (mNSC) on neural stem cells (NSC). METHODS:We quantified the expression of growth factors by mNSC...

journal_title：Cytotherapy

authors： Habisch HJ,Liebau S,Lenk T,Ludolph AC,Brenner R,Storch A

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND AIMS:To date there are only very few data available on the ligamentogenic differentiation capacity of mesenchymal stromal/progenitor cells (MSC) and anterior cruciate ligament (ACL) fibroblasts. METHODS:We describe the in vitro potential of MSC and ACL cells to undergo ligamentogenic differentiation upon tr...

journal_title：Cytotherapy

authors： Haddad-Weber M,Prager P,Kunz M,Seefried L,Jakob F,Murray MM,Evans CH,Nöth U,Steinert AF

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND AIMS:The aim was to evaluate cartilage regeneration in animal models involving induced knee joint damage. Through cell-mediated gene therapy methods, a cell mixture comprising a 3:1 ratio of genetically unmodified human chondrocytes and transforming growth factor beta-1 (TGF-beta1)-secreting human chondrocyt...

journal_title：Cytotherapy

authors： Noh MJ,Copeland RO,Yi Y,Choi KB,Meschter C,Hwang S,Lim CL,Yip V,Hyun JP,Lee HY,Lee KH

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND AIMS:Multiple cell-therapy products require density separation as a part of manufacturing. The traditional method for Ficoll separation, layering cell suspensions over Ficoll in tubes, followed by centrifugation and collection of cells from the interface, is too cumbersome and poses too high a risk of contam...

journal_title：Cytotherapy

authors： Janssen WE,Ribickas A,Meyer LV,Smilee RC

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow (BM) mesenchymal stromal cells (MSC) represent a novel therapy for severe heart failure with extensive myocardial scarring, especially when performed concurrently with conventional revascularization. However, stem cells are difficult to transport in culture media without risk of contaminatio...

journal_title：Cytotherapy

authors： Chin SP,Poey AC,Wong CY,Chang SK,Teh W,Mohr TJ,Cheong SK

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND AIMS:Adoptive transfer of tumor-specific lymphocytes is a promising strategy in the treatment of cancer. We conducted intratumoral administration of an allogeneic irradiated continuous T-cell line (C-Cure 709) expressing an HLA-A2-restricted MART-1-specific T-cell receptor (TCR) into HLA-A2(+) melanoma patie...

journal_title：Cytotherapy

authors： Køllgaard T,Duval L,Schmidt H,Kaltoft K,Seremet T,Andersen MH,Maase Hv,Straten Pt,Hadrup SR

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Mesenchymal stromal cells (MSC) are of clinical interest for their potential use in regenerative medicine and immunotherapy. Originally derived from bone marrow (BM), MSC have now been isolated from most tissues, including umbilical cord (UC) and UC blood (UCB). If MSC from UC are biologically equivalent to ...

journal_title：Cytotherapy

authors： Girdlestone J,Limbani VA,Cutler AJ,Navarrete CV

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Cytokine-induced killer (CIK) cells have shown cytolytic activity against several tumor cells in vitro and in animal tumor models. Furthermore, CIK cells activated by dendritic cell (DC) stimulation show increased anti-tumor activity. This study aimed to evaluate the clinical efficacy of DC-activated CI...

journal_title：Cytotherapy

authors： Li H,Wang C,Yu J,Cao S,Wei F,Zhang W,Han Y,Ren XB

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Enumeration of viable CD34(+) cells provides critical information for the bone marrow (BM) transplant physician. The single-platform ISHAGE protocol is the most reliable method currently available to quantitate accurately this important subset of cells. Previous studies have shown that 5 CD34(+) cells/m...

journal_title：Cytotherapy

authors： Sutherland DR,Nayyar R,Acton E,Giftakis A,Dean S,Mosiman VL

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) have been studied intensively in regenerative medicine. However, their therapeutic potential against tumor formation and cancer metastasis is still unclear. The effects of transplantation of MSCs in early-stage of carcinogenesis, should be evaluated. METHODS:MSC isolated...

journal_title：Cytotherapy

authors： Sun B,Roh KH,Park JR,Lee SR,Park SB,Jung JW,Kang SK,Lee YS,Kang KS

更新日期：2009-01-01 00:00:00

abstract：:Mesenchymal stem cells (MSC) are multipotent cells that differentiate into osteoblasts, myocytes, chondrocytes and adipocytes as well as insulin-producing cells. The mechanism underlying their in vivo differentiation is not clear and is thought to be caused by spontaneous cell fusion or factors present in the microenv...

journal_title：Cytotherapy

authors： Kode JA,Mukherjee S,Joglekar MV,Hardikar AA

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow (BM) stromal cells, also referred to as mesenchymal stromal cells (MSC), can be expanded ex vivo and are able to differentiate along multiple lineages, including chondrocytes, osteoblasts and adipocytes. MSC are known to secrete a number of cytokines and regulatory molecules implicated in di...

journal_title：Cytotherapy

authors： Lazzarotto-Silva C,Binato R,Rocher BD,Costa JA,Pizzatti L,Bouzas LF,Abdelhay E

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Previous studies have shown that nicotine increases endothelial progenitor cell (EPC) numbers and functional activity. However, the mechanisms by which nicotine increases EPC numbers and activity remain to be determined. Recent studies have demonstrated that EPC numbers and activity are associated with ...

journal_title：Cytotherapy

authors： Junhui Z,Xiaojing H,Binquan Z,Xudong X,Junzhu C,Guosheng F

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Human mesenchymal stromal cells (hMSC) are a promising tool for future clinical application, but their use requires rapid cell expansion in media suitable for clinical use. Therefore, we tested the influence of several culture media on colony formation, population doubling (PD) time, cell cycle and surf...

journal_title：Cytotherapy

authors： Turnovcova K,Ruzickova K,Vanecek V,Sykova E,Jendelova P

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Hematopoietic progenitor cells (HPC) circulate at high levels at birth and disappear rapidly afterwards, but the underlying mechanism it is not known. The aim of this study was to assess circulating HPC in cord blood at different gestational ages and shortly after birth and concomitantly study the biologic m...

journal_title：Cytotherapy

authors： Gonzalez S,Amat L,Azqueta C,Madrigal JA,Laïlla JM,Garcia J,Querol S

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Endothelial apoptosis underlies the pathophysiology of pulmonary arterial hypertension (PAH). Some factors/cytokines released by endothelial progenitor cells (EPC) have been revealed as potent inhibitors of apoptosis. The aim of this study was to investigate the effects of EPC on pulmonary microvascular...

journal_title：Cytotherapy

authors： Xia L,Fu GS,Yang JX,Zhang FR,Wang XX

更新日期：2009-01-01 00:00:00

abstract：:There is rapidly increasing interest in the use of magnetic resonance imaging (MRI) to track cell migration in vivo. Iron oxide MR contrast agents can be detected at micromolar concentrations of iron, and offer sufficient sensitivity for T2*-weighted imaging. Cellular MRI shows potential for assessing aspects of cardi...

journal_title：Cytotherapy

authors： Zhang Z,Mascheri N,Dharmakumar R,Li D

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Mesenchymal stromal cells (MSC) have shown diverse therapeutic potential. While characterization of human and mouse MSC has seen significant advances, rat bone marrow-derived MSC (rBM-MSC) remain under-characterized. We detail the isolation, expansion, differentiation, and detailed immunocharacterization of ...

journal_title：Cytotherapy

authors： Harting M,Jimenez F,Pati S,Baumgartner J,Cox C Jr

更新日期：2008-01-01 00:00:00

abstract：:For more than a decade, Ab conjugated to a radionuclide emitting particulate radiation has been used in the management of leukemia in an effort to deliver targeted doses of radiation to BM, spleen and other sites of disease, while sparing normal organs. This radioimmunotherapy (RIT) approach has been employed to achie...

journal_title：Cytotherapy

authors： Pagel JM

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Current efforts to direct differentiation of human embryonic stem cells (hESC) into a particular cell lineage usually lead to a heterogeneous cell population with only a fraction of the desired cell type present. We show the generation of an essentially pure population of human cardiomyocytes from hESC using...

journal_title：Cytotherapy

authors： Xu XQ,Zweigerdt R,Soo SY,Ngoh ZX,Tham SC,Wang ST,Graichen R,Davidson B,Colman A,Sun W

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Mesenchymal stromal cells (MSC) are pluripotent progenitor cells that can be found in human bone marrow (BM). These cells have low immunogenicity and could suppress alloreactive T-cell responses. In the current study, MSC were tested for their capacity to carry and deliver the erythropoietin (EPO) gene in vi...

journal_title：Cytotherapy

authors： Mok PL,Cheong SK,Leong CF,Othman A

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:We have previously demonstrated that bovine dialyzable leukocyte extract (bDLE) induces death through an apoptosis mechanism in MCF-7 breast cancer cells. Depending on the cell type and stimulus, activating protein-1 (AP-1) has been shown to regulate cell proliferation and differentiation, the stress respons...

journal_title：Cytotherapy

authors： Mendoza-Gamboa E,Franco-Molina MA,Zapata-Benavides P,Castillo-Tello P,Vera-García ME,Tamez-Guerra RS,Rodríguez-Padilla C

更新日期：2008-01-01 00:00:00

abstract：:MAb have become an important treatment modality in cancer therapy.Genetically engineered chimeric and humanized Ab have demonstrated activity against a variety of tumors. While the humanized anti-CD33MAb lintuzumab has only modest single-agent activity against overt AML, it can eliminate minimal residual disease detec...

journal_title：Cytotherapy

authors： Jurcic JG

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Retroviral vectors are regularly used to transduce stem cells and their derivatives for experimental and therapeutic purposes. Because these vectors integrate semi-randomly into the cellular genome, analysis of integranated retroviral DNA/host cell DNA junctions (IHJ) facilitates clonality studies of engraft...

journal_title：Cytotherapy

authors： Pule MA,Rousseau A,Vera J,Heslop HE,Brenner MK,Vanin EF

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Bone marrow (BM)-derived mesenchymal stromal cells (MSC) are promising candidate cells for the development of neuroregenerative therapies. We have previously introduced the pro-neural conversion of human MSC to neural stem cell-like cells (m-NSC) by culturing them in suspension culture under serum-free condi...

journal_title：Cytotherapy

authors： Habisch HJ,Fiedler J,Ludolph AC,Storch A,Brenner RE

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Amniotic membrane is a highly promising cell source for tissue engineering. Being part of the placenta, this tissue is abundantly available. It can be processed easily to yield large amounts of epithelial and mesenchymal cells that have shown broad differentiation potential. For tissue-engineering purposes, ...

journal_title：Cytotherapy

authors： Stadler G,Hennerbichler S,Lindenmair A,Peterbauer A,Hofer K,van Griensven M,Gabriel C,Redl H,Wolbank S

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:The possible functional role of basic fibroblast growth factor (bFGF) in regulating the mitotic and metabolic activity of primary human articular chondrocytes was investigated. METHODS:[EF1]Chondrocytes were enzymatically isolated from femoral head cartilage, and were cultured in vitro in monolayer. bFGF-de...

journal_title：Cytotherapy

authors： Schmal H,Zwingmann J,Fehrenbach M,Finkenzeller G,Stark GB,Südkamp NP,Hartl D,Mehlhorn AT

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The molecular mechanisms underlying the biologic effects or differentiation of mesenchymal stromal cells (MSC) have not been clarified. Screening for genes differentially expressed at different stages is an important step in determining these molecular mechanisms. METHODS:In this study, we analyzed the gene...

journal_title：Cytotherapy

authors： Oh I,Ozaki M,Miyazato A,Sato K,Meguro A,Muroi K,Nagai T,Mano H,Ozawa K

更新日期：2007-01-01 00:00:00